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Carry out a blank test. 0 ml. Suitable tests are carried out for validation to demonstrate that the product is non-toxic. 1. System suitability reference solution (b) в thechromatogramshows2clearlyseparatedprincipal spots.

Mobile phase в mobile phase A phosphoric acid R, water R (1999 VV); в mobile phase B acetonitrile R; A1 area of the peak due to echinacoside in the chromatogram obtained with the test solution ; A2 area of the peak due to chlorogenic acid in the chromatogram obtained with the reference solution ; C1 concentration of the test solution, in milligrams per millilitre ; C2 concentration of chlorogenic acid in the reference solution.

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